Amicon centrifugal filter manual

How to Use Amicon® Ultra-4 Centrifugal Filter Devices 1. Add up to 4 mL of sample ( mL if using a 23° fixed-angle rotor) to the Amicon® Ultra filter device. 2. Place capped filter device into centrifuge rotor (swinging-bucket preferred); counterbalance with a similar device. 3. When using a swinging-bucket rotor, s 10–40 minutes. 1 – Maximum centrifugal force. 7, x g (for ultrafiltration) Centrifuge. Fits rotors that work with standard 15 mL conical tubes and are capable of 3, to 14, x g. Sanitization. Non-sterile. May be sanitized by filtering 70% ethanol through the device before use. Macrosep® Centrifugal Filters.  · Amicon Centriprep Manual. How to compare 2 files for differences. General description High recovery centrifugal filters for concentrating samples. Can be used to concentrate biological samples containing antigens, antibodies, enzymes, nucleic acids or micoorganisms. Excellen for use to purify macromolecular components from cell lysates.

Amicon Ultra Centrifugal Filter Devices 11 Direct Weighing Procedure, continued 4. Collect the concentrate with a pipettor and dispense it into the pre-weighed concentrate collection tube. 5. Remove the device from the centrifuge tube and weigh the centrifuge tube and concentrate collection tube. 6. EMD Amicon™ Ultra Centrifugal Filter Units feature regenerated cellulose membranes that are ideal for protein nucleic acid purification, concentration and desalting. High-recovery Ultracel™-PL regenerated cellulose membranes with MWCOs of 3, 10, 30, 50 or kDa. 15 mL starting volume in swinging bucket rotor at x g at 25 °C. Spin times: 3K (40 min); 10K and K (20 min); 30K (10 min); 50K (15 min). Amicon Ultra Centrifugal Filter Unit with Ultracel membrane is CE marked. An invalid quantity was specified. The product quantity has been adjusted.

We’ve engineered Amicon ® Ultra filters with low-binding Ultracel ® regenerated cellulose membranes to ensure more precise molecular weight cut-offs. Amicon ® Ultra centrifugal filters are available in four different volumes: Amicon ® Ultra mL Centrifugal Filters. Amicon ® Ultra 2 mL Centrifugal Filters. How to Use Amicon® Ultra Centrifugal Filter Devices 1. Add up to 15 mL of sample (12 mL if using a fixed-angle rotor) to the Amicon® Ultra filter device. 2. Place capped filter device into centrifuge rotor; counterbalance with a similar device. 3. When using a swinging-bucket rotor, s 15–60 minutes. How to Use Amicon® Ultra-4 Centrifugal Filter Devices 1. Add up to 4 mL of sample ( mL if using a 23° fixed-angle rotor) to the Amicon® Ultra filter device. 2. Place capped filter device into centrifuge rotor (swinging-bucket preferred); counterbalance with a similar device. 3. When using a swinging-bucket rotor, s 10–40 minutes.